Senior Research Fellow, NeuRA
Conjoint Senior Lecturer, UNSW
Janette Mary O’Neil Research Fellow
+612 9399 1836
Jan completed her PhD in human genetics at The University of Melbourne in 2001. She then spent five years as a postdoctoral researcher at The Wellcome Trust Centre for Human Genetics, University of Oxford (UK), where she developed her career interest in psychiatric genetics and complex trait analysis, studying the genetic contributors to personality, depression and anxiety in both humans and mice. She returned to Australia in 2006 on a NHMRC Howard Florey Fellowship, which allowed her to join the institute to study the genetics of bipolar disorder and schizophrenia. In 2013, Jan was promoted to Research Fellow and Group Leader, where she heads a number of projects in both gene discovery, and examining the role of specific genes in risk of major psychiatric conditions.
Despite numerous research into the genetics of psychiatric disorders, investigations regarding the molecular genetics of wellbeing and resilience and in general healthy functioning using new technologies and methods are still scarce. There is very little known about the genetic factors influencing our wellbeing and resilience. Only a few recent genome wide association studies successfully detected a number of genetic variants influencing wellbeing. However, these variants are only responsible for a very small proportion of wellbeing heritability and much more are still waiting to be discovered.
My research area is focused on understanding the role of genetics and environment in mental wellbeing and resilience; in particular, the role of genetic and epigenetic factors and how they interact with each other and the environment in predicting mental health.
We have at our disposal an amazing population of 1600 twins with psychological data including mental health and wellbeing questionnaires, including personality questionnaires and a specific composite wellbeing questionnaire developed by the Gatt group called COMPAS-W. These twins also underwent a genetic analysis using PsychArray and their genotyping data is available. In addition, a portion of these twins have EEG and neuroimaging data (MRI, fMRI, DTI), which will allow us to further investigate the effect of genetic markers on these variables and how they interact to yield the end phenotype.
We have three main questions to answer in this project:
First, do genetic variations influence wellbeing in our twin cohort? Which genes? How and to what extent?
Second, do genetic variations influence the neurobiological markers measured by EEG, MRI, fMRI and DTI in the twin population?
Third, is there any connection or correlation between the genetic markers which influence wellbeing and those which influence the neurobiological markers? How much of these phenotypes are genetically correlated?
The offspring of individuals with bipolar disorder are at increased risk of mental illness, but our tools to predict which of these genetically at-risk young people will eventually develop disorder are very imprecise. Longitudinal studies that ascertain at-risk participants and monitor them prospectively are an effective approach for identifying early clinical and biological markers of future illness. In collaboration with the Black Dog Institute plus groups from four independent US-based sites, including: Johns Hopkins University; University of Michigan; Washington University in St. Louis; Indiana University; we are following a cohort of young kids and siblings of bipolar disorder patients with annual clinical, neurocognitive and lifestyle assessments; plus bi-annual brain imaging of the Australian participants. We are assessing the genetic load of multiple risk variants across the genome in these at-risk individuals to determine if we can use genetic information to help predict which individuals will ultimately transition to illness, and whether genetic load will influence early structural brain changes which are seen prior to onset of symptoms which lead to a clinical diagnosis.
We are also examining whether epigenetic changes – which occur on-top-of the DNA sequence in response to environmental influences – are involved in transition from health to illness. Early identification of those most likely to develop illness will provide a firm basis on which to develop preventive and early intervention strategies to reduce the impact of this devastating disorder.
Together with Professor Peter Schofield (NeuRA) and Professor Philip Mitchell (Black Dog Institute), our group is investigating the genetic contributors to bipolar disorder using Australian families with multiple individuals who have been diagnosed with the disorder.
The group previously identified a bipolar susceptibility locus located on chromosome 15 in a pooled analysis of 35 families. More detailed analysis of this region has identified a single gene, which confers an increased susceptibility to both bipolar disorder and schizophrenia, and has also been implicated as a risk factor for autism.
The group is now aiming to understand how alterations in ST8SIA2 translate into an increased genetic susceptibility by characterising alterations in the DNA, RNA and protein product of this gene and its interaction partners in patients with either bipolar disorder or schizophrenia.
Because of the complex pattern of genetic transmission, it is expected that multiple genes will contribute to susceptibility to bipolar disorder. It is possible that combinations of genes will be stronger risk factors for developing bipolar disorder than individual genes, so we are examining gene-gene interactions (genetic epistasis) throughout the genome to identify genes which, in concert, may increase susceptibility. This analysis has led to the identification of multiple such interacting regions, and the group is now seeking to identify the specific genes involved in these interactions.
Recent advances in technology have enabled sequencing at the level of the entire genome to become a reality. We have access to number of rare families with highly heritable forms of bipolar disorder, for which we will apply this powerful technology to identify specific genetic factors which increase disease risk. We will assess loss-of-function variation within genes, at both the level of single base mutations and variation in gene copy number, which track with illness in these families to identify new genes which contribute to illness.
In addition to genes identified in our own laboratory, we have also been involved in assessing the risk attributed by genes identified by other groups and in sharing data and samples in large international collaborative studies. We are contributors to the Psychiatric Genomics Consortium (PGC) and Enhancing Neuroimaging Genetics through Meta-Analysis (ENIGMA) consortia, which aim to identify risk genes which contribute to disease, and examine their effect on brain structure, function and disease.
Genetic research into bipolar disorder traditionally uses strict categorical criteria to define a clinical diagnosis. However, it is common for relatives of individuals with bipolar to exhibit some evidence of mood disturbance, but not sufficient to meet the strict clinical criteria for a positive diagnosis. These individuals are correctly considered clinically unaffected, although they likely share some of the susceptibility genes underlying the disorder. Dr Fullerton has been examining the use of subclinical traits to identify individuals who share susceptibility genes in order to follow the pattern of genetic transmission of bipolar disorder through families more accurately.
Both genetic and environmental factors are involved in the development of bipolar disorder, a severe mood disorder characterised by oscillations from normal mood to periods of elevated mood (mania) or low mood (depression). Dr Jan Fullerton and Professor Peter Schofield are investigating the genetic contributors to bipolar disorder using Australian families with multiple individuals who have been diagnosed with the disorder. Together with PhD student Erica McAuley, the group has recently identified a bipolar susceptibility locus located on chromosome 15 in a pooled analysis of 35 families. Further, more detailed analysis of this region using a newly acquired campus Illumina beadstation facility for the analysis of SNP markers has identified a single gene, which confers an increased susceptibility to bipolar disorder. They are now aiming to understand how these alterations translate into an increased genetic susceptibility by characterising the biological pathways involved.
Because of the complex pattern of genetic transmission, they expect that multiple genes will contribute to susceptibility to bipolar disorder. It is possible that combinations of genes will be stronger risk factors for developing bipolar disorder than individual genes, so they are examining gene-gene interactions (genetic epistasis) throughout the genome to identify genes which, in concert, may increase susceptibility. This analysis has led to the identification of multiple such interacting regions, and they are now seeking to identify the specific genes involved in these interactions.
ANNA HEATH Research Assistant
KERRIE PIERCE Senior Research Assistant
: +61 2 9399 1883